Fig 5. Neutralization of IL-6 did not prevent enhanced IL-17A production by CD4⁺ T cells cultured with type II macrophage.

Macrophages were stimulated as described in Fig 2 in the presence of αIL-6 (MP5-20F3, 2 μg/ml) or isotype control (rat IgG₁, 2 μg/ml). After four hours, purified CD4⁺ 2D2 T cells and MOG_35-55 (25 μg/ml) were added to the macrophage cultures for 72 hours. IFN-γ (a), IL-17A (b), and IL-6 (c) were measured by ELISA. Shown are the means from 2 independent experiments (c) and means and SEM of triplicate wells from a representative experiment of 2–3 independent experiments (a & b). *p<0.05, **p<0.01, and ***p<0.001 by one-way ANOVA with Newman-Keuls’ post-test.