Fig 1. CFTR expression in airway epithelial cells.

(A, B) Levels of mRNA (A), and protein (B) in human bronchial epithelial cells expressing wild type (WT) or F508del CFTR. Cells were grown on plastic, as polarizing cell monolayers on permeable supports using an air/liquid (A/L) interface, or on the same supports with overlying liquid (liquid/liquid, L/L). Mature, fully glycosylated CFTR (Band C), and the ER localized glycoform (Band B) are depicted for air/liquid interface culture, which is most representative of physiologic conditions. (C, D) Steady state levels of CFTR mRNA (C) and protein (D) in primary airway epithelial cells grown at an air/liquid interface and isolated from non-CF or F508del homozygous individuals. mRNA levels were normalized against CFBE cells expressing WT CFTR (A) or non-CF human primary airway cells (C) in fold difference. N ≥ 3 samples per condition. Representative western blots in (B) and (D) are shown.