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. 2016 Oct 12;11(10):e0164544. doi: 10.1371/journal.pone.0164544

Fig 4. Tomosyn forms SNARE complexes with SNAP25 and syntaxin 1 in the posterior pituitary.

Fig 4

(a–c) Coimmunoprecipitation assays were performed using protein G Sepharose and mouse immunoprecipitation (IP) antibodies (a), protein A Sepharose and rabbit IP antibody (b) or ImmunocruzE™ imaging system (Santa Cruz Biotechnology, Santa Cruz, CA, USA) and mouse IP antibody (c), respectively. Eluted samples were subjected to SDS-PAGE followed by western blotting using anti-tomosyn, anti-SNAP25, anti-syntaxin 1A, or anti-syntaxin 1B antibodies (arrowheads at right). Input was 1/200 (volume) of the total rat posterior pituitary lysates used for the assay. CTR = IP with anti-IgG antibody.