Table 1. List of primary antibodies.
Protein | Catalog No | Poly/Mono | Host | IB | IHC | ICC | IP | Company |
---|---|---|---|---|---|---|---|---|
Tomosyn | sc-136105 | Mono | Mouse | 1:200 | 1:50 | 5μg | Santa Cruz Biotechnology | |
ab41271 | Poly | Rabbit | 1:1000 | 1:1000 | 1:100 | Abcam | ||
SNAP25 | ab5666 | Poly | Rabbit | 1:1000 | 1:50 | Abcam | ||
ab41455 | Poly | Rabbit | 1:20 | Abcam | ||||
111011 | Mono | Mouse | 1:1000 | 5μg | Synaptic Systems | |||
Syntaxin1A | 110111 | Mono | Mouse | 1:2000 | 5μg | Synaptic Systems | ||
110302 | Poly | Rabbit | 1:200 | Synaptic Systems | ||||
Syntaxin1B | 110403 | Poly | Rabbit | 1:1000 | 1:1000 | 5μg | Synaptic Systems | |
Copeptin | sc-7812 | Poly | Goat | 1:100 | 1:100 | Santa Cruz Biotechnology | ||
AVP | AB1565 | Poly | Rabbit | 1:1000 | CHEMICON | |||
β-actin | A2228 | Mono | Mouse | 1:10000 | Sigma | |||
Akt | 4691 | Mono | Rabbit | 1:10000 | Cell Signaling | |||
N-cadherin | 610920 | Mono | Mouse | 1:2000 | BD Bioscience | |||
normal mouse IgG | sc-2025 | Mono | Mouse | 5μg | Santa Cruz Biotechnology | |||
normal rabbit IgG | PP64 | Poly | Rabbit | 5μg | Millipore | |||
NeuN | MAB377 | Mono | Mouse | 1:50 | Millipore |
Poly/Mono = polyclonal or monoclonal antibody; IB = immunoblot dilutions used; IHC = immunohistochemistry dilutions used; ICC = immunocytochemistry dilutions used; IP = immunoprecipitation total protein used.