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. Author manuscript; available in PMC: 2016 Oct 12.
Published in final edited form as: ACS Synth Biol. 2015 Jun 8;4(10):1124–1135. doi: 10.1021/acssynbio.5b00048

Figure 5.

Figure 5

CRY2 oligomerization on the ER membrane can be differentially suppressed by CRY2-CIB1 heterodimerization. Cells were illuminated with a single blue light pulse of 2 s. (a) Cotransfection of COS-7 cells with GFP-CIB1 and CRY2-mCh-Sec61 does not visibly affect CRY2 cluster formation on the ER membrane. (b) Coexpression of RAF-GFP-CIB1 and CRY2-mCh-Sec61 moderately reduces CRY2 cluster formation. The number and the intensity of clusters are reduced as compared with cells singly transfected with CRY2-mCh-Sec61. (c) Coexpression of GFP-BICDN-CIB1 and CRY2-mCh-Sec61 completely blocks CRY2 cluster formation, where no CRY2 cluster is visible after exposure to blue light stimulation. (d) Quantitative analysis of cluster formation in COS-7 cells cotransfected with CRY2-mCh-Sec61 and various CIB1 plasmids shows that CRY2 oligomerization can be suppressed at variable degrees. Error bars represent standard deviation of the mean. Scale bars, 10 µm.