Table 2.

Sex ratios of transgenic and non-transgenic progeny of five transgenic lines*.

DOI: http://dx.doi.org/10.7554/eLife.19281.006

Line	Transgenic male	Transgenic female	Negative male	Negative female
nGuy1-1 †	71	0	58	56
nGuy1-2 †	129	0	91	83
Guy1m †	69	62	26	34
bGuy1C-1 ‡	78	0	75	66
bGuy1N-1 ‡	77	0	66	65

Notes:.

*Crosses were done between the heterozygous transgenic males and wildtype females. Screening and sexing was initially done at the L3 instar stage. The sexed negative and positive larvae were reared separately to adulthood and sex was further confirmed on the basis of antennae morphology. The total numbers of each sex for the transgenic and non-transgenic groups are listed.

^†The numbers for these lines are from generation 7 (G₇). Note that Guy1m has a point mutation that abolished the Guy1 open reading frame. There are more transgenic individuals than non-transgenic individuals in the Guy1m line because both transgenic females and wild type females were mated with transgenic males to maintain the line.

^‡Two bGuy1C lines and nine bGuy1N lines were obtained and only one of each is shown in this Table. The numbers are from generation 4 (G₄).