Figure 9. TP53RTG proteins are unlikely to directly regulate TP53 target genes.
(A) Domain structure of TP53 and TP53RTG proteins. (B) Localization of TP53RTG12-GFP in African elephant fibroblasts. (C) Relative luciferase (Luc.) expression in African elephant fibroblasts transfected with co-transfected with either the TP53RTG12 pcDNA3.1(+)/myc-His expression vector and the pGL4.38[luc2P/p53 RE/Hygro] luciferase reporter vector, or empty pcDNA3.1(+)/myc-His and the pGL4.38[luc2P/p53 RE/Hygro] luciferase reporter vector. Data are shown as fold difference in Luc. expression 48 hr after transfection standardized to Renilla and cells transfected with pcDNA3.1(+)/myc-His and pGL4.38[luc2P/p53 RE/Hygro]. n = 10.