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. Author manuscript; available in PMC: 2017 Nov 1.

Published in final edited form as: Biochem Pharmacol. 2016 Aug 26;119:93–104. doi: 10.1016/j.bcp.2016.08.021

Fig. 6 — Multivariate data analysisand metabolite identification in Ahr^+/+ and Ahr^-/- mice by using UPLC ESI QTOFMS analysis. Ahr^+/+ and Ahr^-/- mice were treated with vehicle or FLU (200mg/kg) for 28 days. (A) Scores plot of serum metabolome in WT mice treated with vehicle ( ) and FLU ( ) as determined by PCA. (B) Scores plot of serum metabolome in Ahr^-/- mice dosed with vehicle ( ) and FLU ( ) as determined by PCA. (C) S-plot of OPLS-DA recognized serum metabolome in vehicle- and FLU-treated Ahr^+/+ mice, in which identified metabolites were indicated. (D) S-plot of OPLS-DA recognized serum metabolome in vehicle- and FLU-treated Ahr^-/- mice. Each point represents an individual mouse serum sample (A, B) and a unique ion (C, D). The t[1] and t[2] represent principal components 1 and 2, respectively. The p(corr)[1] represents the interclass difference, and p[1] represents the relative abundance of the ions. (E-H) Quantitation of bile acids in Ahr^+/+ and Ahr^-/- mice. (E) Individual bile acids in serum of Ahr^+/+ mice. (F) Individual bile acids in serum of Ahr^-/- mice. (G) Individual bile acids in livers of Ahr^+/+ mice. (H) Individual bile acids in livers of Ahr^-/- mice. Data are presented as mean ± SEM; n = 6/group. *P < 0.05, or **P < 0.01, versus vehicle group, by two-tailed Student’s t-test.

Inline graphic — Multivariate data analysisand metabolite identification in Ahr^+/+ and Ahr^-/- mice by using UPLC ESI QTOFMS analysis. Ahr^+/+ and Ahr^-/- mice were treated with vehicle or FLU (200mg/kg) for 28 days. (A) Scores plot of serum metabolome in WT mice treated with vehicle ( ) and FLU ( ) as determined by PCA. (B) Scores plot of serum metabolome in Ahr^-/- mice dosed with vehicle ( ) and FLU ( ) as determined by PCA. (C) S-plot of OPLS-DA recognized serum metabolome in vehicle- and FLU-treated Ahr^+/+ mice, in which identified metabolites were indicated. (D) S-plot of OPLS-DA recognized serum metabolome in vehicle- and FLU-treated Ahr^-/- mice. Each point represents an individual mouse serum sample (A, B) and a unique ion (C, D). The t[1] and t[2] represent principal components 1 and 2, respectively. The p(corr)[1] represents the interclass difference, and p[1] represents the relative abundance of the ions. (E-H) Quantitation of bile acids in Ahr^+/+ and Ahr^-/- mice. (E) Individual bile acids in serum of Ahr^+/+ mice. (F) Individual bile acids in serum of Ahr^-/- mice. (G) Individual bile acids in livers of Ahr^+/+ mice. (H) Individual bile acids in livers of Ahr^-/- mice. Data are presented as mean ± SEM; n = 6/group. *P < 0.05, or **P < 0.01, versus vehicle group, by two-tailed Student’s t-test.