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. Author manuscript; available in PMC: 2017 Jun 1.
Published in final edited form as: Acta Biomater. 2016 Mar 24;37:120–130. doi: 10.1016/j.actbio.2016.03.036

Figure 2. Heparin washing procedure successfully removes all bound plasmid.

Figure 2

Following an incubation for 1 hour at 4°C, 50 µg/mL heparin in PBS was used to eliminate all associated DNA. Cy3-labeled plasmid is removed from both cells (with added polyplex) and nuclei (with added plasmids). NGM indicated normalized geometric mean fluorescence (unitless).