Fig. 3.

mtDNA absolute quantitation from rat retinal synaptosomes. a Somatic and synaptic retinal cell fractions were used to determine the absolute quantitation of mtDNA. Somatic cell fraction contained both nuclear and mtDNA molecules. Synaptic fraction only contained mtDNA molecules with an insignificant amount of nuclear. b Representative western blots on nuclear, cytoplasmic, and synaptosome fractions probed for Lamin B1 (66–70 kDa), a nuclear marker, Synaptophysin (38 kDa), a pre-synaptic terminal marker, and COXIV (15–16 kDa), a mitochondrial marker