Figure 2.

Purification of FtsX‐His. SDS‐PAGE: lane 1, cobalt column affinity‐purified FtsX‐His; Lanes 2–5, fractions from gel filtration after affinity‐purified FtsX‐His was loaded on a Hi Load Superdex 200 16/60 column that was equilibrated and eluted with 50 mmol/L Tris‐HCl, pH 8.0, 200 mmol/L NaCl, 5% (vol/vol) glycerol, 0.02% (wt/vol) DDM, and 0.02% (wt/vol) C₁₂E₈. The FtsX‐His band at 34 kDa and the standards ladder are indicated in the SDS‐PAGE gel stained with Coomassie blue. Overexpression, affinity purification, gel filtration, and storage of FtsX‐His are described in Materials and Methods.