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. 2016 Jun 28;5(5):802–818. doi: 10.1002/mbo3.371

Figure 5.

Figure 5

The folC2 gene was essential for colitis attenuation and anti‐inflammatory activity of L. reuteri 6475 in vivo. (A) Supernatant from L. reuteri 6475 significantly decreased weight loss in mice challenged with intrarectal TNBS, whereas 6475::folC2 did not have such effects. Differences in weight loss are shown as percent weight loss 48 h after induction of TNBS colitis. (B) Supernatant from L. reuteri 6475 significantly decreased colonic macroscopic injury in mice challenged with TNBS, whereas 6475::folC2 did not have such effects. Differences in colonic macroscopic injury are shown as Wallace score. Data presented as mean ± SEM. (C) Supernatant from L. reuteri 6475 significantly decreased plasma SAA concentrations in mice challenged with TNBS, whereas 6475::folC2 did not have such effects. Plasma levels of SAA, an indicator of inflammation, were measured by ELISA. N = 19, 50, 47, and 26 for colitis‐negative, colitis‐positive, ATCC 6475, and 6475::folC2, respectively. Statistical analyses were performed using GraphPad Prism (GraphPad Inc., La Jolla,CA). Data were presented using box and whisker plots showing the median and 5th and 95th percentiles. Statistical significance was assessed by nonparametric Kruskal–Wallis test. Differences between experimental groups are reported as mean fold difference ± SEM, ***P < 0.001, **P < 0.01, *P < 0.05.SAA, Serum amyloid A; TNBS, trinitrobenzene sulfonic acid.