Table 3.
Comparison of calculated time to achieve 50% activity (t50) for membrane depolarization, membrane permeabilization, and cell survival
| Protein | Membr. depolarization t50 (sec) | Membr. permeabilization t50 (sec) | Cell survival t50 (sec) | Cell survival (%) |
|---|---|---|---|---|
| RNase 7 | 261.23 | 595.53 | 1397 | 38.2 |
| RNase 7‐H15A | 288.53 | 698.15* | 1763* | 54.86** |
| RNase 3 | 251.36 | 490.84 | 2354 | 56.87 |
| RNase 3‐H15A | 356.75* | 975.5** | 2965* | 67.89** |
All assays were carried out at 1 μmol/L final protein concentration. Depolarization was assayed using DISC3(5) dye, cell leakage by the Sytox Green assay and survival percentage at final incubation time (120 min) was evaluated using the Live/dead® kit. T‐student was applied for comparison of numerical variables using as reference each activity corresponding to wild‐type protein, where * corresponds to P < 0.05 and ** to P < 0.009.