Table 2.
Identification of proteins predominantly affected in C. jejuni Bf under aerobic conditions.
| Protein ID | Name | Gene | ANOVAa (p-value) | Foldb | Theoretical MW (Da)/pI | Log E-valuec | No. of MP/TP (Pc)d |
|---|---|---|---|---|---|---|---|
| CELL PROCESS | |||||||
| Detoxification | |||||||
| tr|Q0PBH5 | Alkyl hydroperoxide reductase | AhpC | 6.27E-03 | +2.0 | 21.9/5.66 | −110.26 | 25/75 (84%) |
| 2.03E-02 | +1.8 | −82.37 | 13/171 (65%) | ||||
| 6.01E-03 | +2.5 | −88.39 | 22/46 (57%) | ||||
| sp|Q59296 | Catalase | KatA | 1.08E-02 | +1.7 | 58.29/7.74 | −102.42 | 22/53 (46%) |
| sp|Q9PPE0 | Probable thiol peroxidase | Tpx | 7.25E-03 | +1.7 | 18.40/5.13 | −63.49 | 8/22 (47%) |
| tr|Q0PBZ1 | Thioredoxin reductase | TrxB | 3.92E-02 | +1.5 | 33/5.60 | −92.07 | 11/60 (57%) |
| 3.55E-03 | +2.8 | −65.10 | 9/22 (37%) | ||||
| Chaperones, chaperonins, starvation | |||||||
| sp|O69289 | 60 kDa chaperonin | GroL | 7.39E-03 | −1.8 | 57.79/5.02 | −279.73 | 40/187 (65%) |
| 1.42E-02 | +2.0 | −33.35 | 6/10 (15%) | ||||
| 1.07E-02 | +2.1 | −7.86 | 2/3 (6%) | ||||
| 5.46E-03 | +1.5 | −211.64 | 31/50 (52%) | ||||
| 4.42E-02 | −2.2 | −186.05 | 34/51 (58%) | ||||
| 2.99E-02 | −1.7 | −227.73 | 34/89 (59%) | ||||
| sp|Q0P891 | DNA protection during starvation protein | Dps | 2.56E-04 | +2.9 | 17.1/5.55 | −13.89 | 4/4 (34%) |
| sp|Q9PI02 | Chaperone protein | ClpB | 7.25E-03 | +1.7 | 95.40/5.47 | −110.17 | 27/46 (34%) |
| ClpB | 1.40E-02 | +1.7 | −62.96 | 16/20 (22%) | |||
| sp|O69298 | Chaperone protein DnaK | DnaK | 2.46E-02 | +1.6 | 67.30/4.97 | −270.16 | 48/85 (50%) |
| Transport/binding proteins | |||||||
| tr|Q0P7X0 | Periplasmic protein | p19 | 1.15E-03 | +1.5 | 19.6/4.97 | −97.57 | 16/70 (64%) |
| 1.05E-02 | −1.5 | −39.82 | 5/77 (50%) | ||||
| tr|Q0P8K8 | Putative periplasmic protein | Cj1406c | 4.13E-02 | +2.3 | 24.9/5.89 | −17.77 | 8/9 (39%) |
| tr|Q0P8M9 | Peb1A major cell-binding factor | Peb1A | 6.10E-03 | +1.5 | −46.86 | 13/14 (45%) | |
| tr|Q0PAQ4 | Putative periplasmic phosphate binding protein | PstS | 1.58E-04 | −2.3 | 36/5.76 | −111.37 | 20/70 (58%) |
| tr|Q0PBW4 | Putative iron-uptake ABC transport system periplasmic iron-binding protein | CfbpA | 4.08E-03 | +2.1 | 37.4/8.97 | −111.73 | 20/86 (57%) |
| tr|Q0P9N4 | Branched-chain amino-acid ABC transport system periplasmic binding protein | LivK | 1.95E-02 | +2.0 | 39.59/8.70 | −86.36 | 11/17 (35%) |
| 3.76E-02 | +2.2 | −86.38 | 11/35% | ||||
| SMALL MOLECULE METABOLISM | |||||||
| Energy metabolism - tricarboxylic acid cycle | |||||||
| sp|O69294 | Fumarate hydratase | FumC | 1.39E-03 | +1.9 | 50.59/6.12 | −138.94 | 27/109 (51%) |
| 1.61E-03 | +1.7 | −156.55 | 30/48 (53%) | ||||
| tr|Q0PBA1 | Fumarate reductase flavoprotein subunit | FrdA | 6.03E-04 | +2.2 | 73.59/6.36 | −136.96 | 27/71 (44%) |
| 2.32E-04 | +2.2 | −121.49 | 25/42 (43%) | ||||
| tr|Q0P8X0 | Malate oxidoreductase | Mez | 1.82E-03 | +2.4 | 43.90/5.78 | −94.02 | 16/49 (40%) |
| tr|Q0P7U8 | Citrate synthase | GltA | 1.49E-04 | +3.1 | 47.90/6.47 | −80.33 | 17/42 (48%) |
| tr|Q0PA55 | Aconitate hydratase 2 | AcnB | 6.73E-03 | −1.6 | 92.59/6.04 | −144.40 | 27/82 (41%) |
| 1.97E-02 | −1.6 | −196.23 | 32/117 (46%) | ||||
| 2.30E-02 | −1.5 | −222.33 | 37/142 (50%) | ||||
| tr|Q0PAY0 | 2-oxoglutarate:acceptor oxidoreductase | OorA | 5.26E-03 | +1.8 | 40.90/6.12 | −74.90 | 17/37 (42%) |
| ATP-proton motive force | |||||||
| sp|Q0PC30 | ATP synthase subunit beta | AtpD | 1.77E-02 | +1.5 | 50.70/4.97 | −137.76 | 25/127 (59%) |
| Lipid metabolism; fatty acid biosynthesis | |||||||
| tr|Q0PB68 | 3-oxoacyl-[acyl-carrier-protein] synthase 2 | FabF | 3.54E-02 | −1.5 | 42.59/5.64 | −180.60 | 29/103 (58%) |
| 1.60E-02 | −1.5 | −74.51 | 12/146 (53%) | ||||
| Lipopolysaccharide biosynthesis | |||||||
| tr|Q0P9A5 | ADP-L-glycero-D-manno-heptose-6-epimerase | HldD | 3.59E-04 | −1.7 | 35.90/6.12 | −138.21 | 23/73 (69%) |
| CENTRAL INTERMEDIARY METABOLISM | |||||||
| Amino acid biosynthesis | |||||||
| sp|P71128 | Cysteine synthase B | CysM | 6.28E-04 | −1.8 | 32.30/6.34 | −82.93 | 16/67 (57%) |
| 6.72E-03 | −2.2 | −19.81 | 3/21 (21%) | ||||
| tr|Q0PAU2 | Acetolactate synthase small subunit | IlvH | 1.48E-02 | +1.8 | 17.3/6.75 | −26.11 | 8/10 (52%) |
| Biosynthesis of secondary metabolites | |||||||
| tr|Q0P9F8 | S-adenosylmethionine synthetase | MetK | 4.04E-02 | −1.6 | 43.7/5.45 | −103.70 | 19/63 (56%) |
| 6.50E-03 | −1.9 | −126.01 | 25/86 (55%) | ||||
| 3.21E-02 | −1.9 | −142.72 | 27/102 (59%) | ||||
| BROAD REGULATORY FUNCTIONS | |||||||
| Signal transduction | |||||||
| tr|Q0PB65 | Putative MCP-type signal transduction protein | Cj0448c | 3.17E-02 | +1.6 | 40.40/5.58 | −80.75 | 15/55 (47%) |
| Synthesis of autoinducer 2 | |||||||
| sp|Q9PN97 | S-ribosylhomocysteine lyase | LuxS | 7.25E-03 | +1.7 | 18.10/6.19 | −38.61 | 7/16 (50%) |
a
Normalized spots were compared for their abundance between aerobic and microaerobic conditions. Significant difference is indicated for each spot.
b
Positive values correspond to the fold of higher abundance in aerobic conditions and negative values correspond to the fold of lower abundance in aerobic conditions for each spot.
C
Each protein was identified with a mass tolerance < 20 ppm and at least two peptides.
d
No. of MP/TP (Pc): Number of matched/total peptides (Protein coverage).