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. 2016 Oct 13;6:126. doi: 10.3389/fcimb.2016.00126

Figure 2.

Figure 2

SpyB deficiency causes MGAS5005 cells to aggregate. MGAS5005, 5005ΔspyB, 5005ΔspyB spyB+, and 5005ΔspyA strains at middle exponential phase were examined by microscopy. (A) MGAS5005 strains were Gram stained and imaged by light microscopy. (B) MGAS5005 strains were stained with fluorescently labeled vancomycin to visualize the cell walls and septa, and imaged by fluorescence microscopy. (C) Fixed cultures of MGAS5005 and 5005ΔspyB were visualized by transmission electron microscopy or (D) scanning electron microscopy.