Figure 5.

Bcl11b deficiency does not affect T-bet and Eomes expression levels in VacV-specific CD8 T cells. WT (Bcl11b^flox/flox/dLck-iCre⁻) or Bcl11b-conditional knockout (Bcl11b^flox/flox/dLck-iCre⁺) mice were infected i.p with VacV-WR (2 × 10⁵ PFU/mouse). Eight days postinfection, splenocytes and lung cells were harvested and subsequently stained for CD8α, CD44, T-bet, Eomes, and B8R_20–27/k^b tetramer. Representative plots for T-bet (A) and Eomesodermin (B) staining gated on CD8⁺CD44⁺ B8R_20–27/K^b-tetramer⁺ cells recovered from the spleen and lung as indicated. Numbers indicate mean fluorescence intensity (MFI) within the gated population. Gates were based on controls, after gating on CD44^lo cells (Gray shaded area) in the same host. The p-values are <0.05 by two-tailed Student t-test (WT vs. Cko).