Table 1.
Strains and plasmids used in this study.
| Strain | Properties | References |
|---|---|---|
| B. subtilis 168 | trpC2 | Kunst et al., 1997 |
| B. subtilis 168 ΔspoVT | spoVT::specr | Bagyan et al., 1996, kindly provided by Prof. S. Cutting |
| B. cereus ATCC 14579 | Enterotoxic strain of B. cereus wild type isolate | Bacillus Genetic Stock Center, ATCC, BGSC ID6A5 |
| B. cereus ATCC 14579 PspoVA-gfp | PspoVAA-gfp, Cmr | This study |
| B. cereus ATCC 14579 ΔspoVT | spoVT::specr | This study |
| B. cereus ATCC 14579 ΔspoVT PspoVA-gfp | spoVT::specr, PspoVAA-gfp, Cmr | This study |
| B. cereus ATCC 14579 ΔBC1117 | marker-less deletion of the BC1117 ORF | This study |
| Plasmid | Properties | References |
| pNW33n | E. coli–Gram + shuttle vector | Bacillus Genetic Stock Center |
| pNWVT | Cmr, spoVTBCE | This study |
| pVTBsu2 | Cmr, spoVTBSU | This study |
| pSG1151 | Vector for integrative P-gfpmut1 fusions in B. subtilis, Apr, Cmr | Lewis and Marston, 1999 |
| pSGCVA | pSG1151 with gfp driven by the Bce spoVAA promoter, Apr, Cmr | This study |
| pMAD | Vector for efficient gene replacement in non-naturally transformable gram-positive bacteria, Apr, Emr | Arnaud et al., 2004 |
| pDCVT | pMAD-derivative used for spoVT gene disruption, Apr, Emr | This study |
| pDG1726 | E. coli plasmid bearing a spectinomycin resistance cassette | Guérout-Fleury et al., 1995 |