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. 2016 Oct 13;7:456. doi: 10.3389/fphys.2016.00456

Figure 1.

Figure 1

Osteocalcin genes and their receptors in different tissues of 3 months old female and male mice. Relative expression levels of the osteocalcin genes in the tissues of 3 months old female and male mice. The genes under investigations were: the osteocalcin Bglap gene and its receptor Gprc6a and the gamma-glutamyl carboxylase Ggcx gene. The tissues under investigations were: brain, bone, white adipose tissue (WAT), brown adipose tissue (BAT), uterus, ovaries, and testis. Brain and bone data were reported for comparison. Bone has to be considered the positive control of Bglap gene expression. In each graph, the bars are the means ± SEM from the n animals indicated in brackets. For the Gprc6a gene, 2 out of 5 and 4 out of 7 analyzed samples from female and male mice, respectively, were not amplified due to the extremely low expression levels of this gene in all tissues except testis. Differences were found within groups and between groups as determined by one way analysis of variance for Bglap, Gprc6a, and Ggcx. Data significantly different (P < 0.05) vs. brain (◦), bone (*), WAT (#), and BAT (•) using Bonferroni analysis.