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. 2016 Oct 13;7:363. doi: 10.3389/fphar.2016.00363

FIGURE 2.

FIGURE 2

Comparison of MRD in three HCL cases by qualitative PCR for IgH rearrangement, QT-PCR, and dd-PCR. (A) Patient achieved the CR after 3 months and maintained it at the end of treatment. QT-PCR was negative already at the first point when dd-PCR and IgH rearrangement were positive. The mutational burden measured by dd-PCR reduced from 4.87 at diagnosis to 2 at 3 months and became negative at the end of treatment. IgH rearrangement also became negative at the end of therapy. (B) Patient achieved the PR after 3 months and the CR at the end of treatment. The IgH rearrangement remained clonal also when CR was achieved. The mutational burden measured by dd-PCR reduced from 4 at diagnosis to 2 at 3 months when response was partial. At this time point, QT-PCR was already negative. (C) Patient achieved the PR after 3 months but progressed at the end of treatment. IgH was positive both at 3 months and at the end of therapy. QT-PCR mutational burden reduced at the first time point and increased from 0.1 to 9.15 at the sixth month. The mutational burden measured by dd-PCR decreased from 1 at diagnosis to 0.01 at 3 months and increased up to 1.4 at the end of treatment.