TABLE 2 .
LOH quantification on Chr4 by flow cytometry
| Strain | Cellpopulation | Growthcondition | na | LOH frequency± SEM (×10−4) | Foldchange | Mann-Whitneytest P value |
|---|---|---|---|---|---|---|
| I-SceI+TargetB | Mono-GFP | YPD | 37 | 1.35 ± 0.1 | 30 | ≤0.0001 |
| YPD + ATc | 40 | 40.0 ± 0.9 | ||||
| Mono-BFP | YPD | 37 | 1.0 ± 0.1 | 17 | ≤0.0001 | |
| YPD + ATc | 40 | 16.8 ± 0.6 | ||||
| I-SceI+TargetA | Mono-GFP | YPD | 36 | 2.0 ± 0.1 | 9 | ≤0.0001 |
| YPD + ATc | 36 | 18.7 ± 0.3 | ||||
| Mono-BFP | YPD | 36 | 1.8 ± 0.1 | 48 | ≤0.0001 | |
| YPD + ATc | 36 | 85.5 ± 1.6 | ||||
| I-SceI+TargetB+GPI16 | Mono-GFP | YPD | 36 | 0.3 ± 0.02 | 105 | ≤0.0001 |
| YPD + ATc | 36 | 31.6 ± 2.2 | ||||
| Mono-BFP | YPD | 36 | 0.5 ± 0.04 | 58 | ≤0.0001 | |
| YPD + ATc | 36 | 29.0 ± 1.3 | ||||
| I-SceI+TargetA+GPI16 | Mono-GFP | YPD | 36 | 0.3 ± 0.02 | 43 | ≤0.0001 |
| YPD + ATc | 35 | 14.8 ± 0.9 | ||||
| Mono-BFP | YPD | 36 | 1.0 ± 0.1 | 100 | ≤0.0001 | |
| YPD + ATc | 35 | 101.8 ± 1.6 | ||||
| I-SceI only | Mono-GFP | YPD | 21 | 1.1 ± 0.1 | 1 | 0.9424 |
| YPD + ATc | 21 | 1.1 ± 0.1 | ||||
| Mono-BFP | YPD | 21 | 1.0 ± 0.0 | 1 | 0.4605 | |
| YPD + ATc | 21 | 0.9 ± 0.0 | ||||
| Target only | Mono-GFP | YPD | 21 | 1.0 ± 0.1 | 1 | 0.0514 |
| YPD + ATc | 21 | 0.9 ± 0.1 | ||||
| Mono-BFP | YPD | 21 | 1.1 ± 0.1 | 1 | 0.2251 | |
| YPD + ATc | 21 | 1.0 ± 0.1 |
a
Each value represents the number of biological replicates analyzed.