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(a) Preparation of 3D collagen scaffolds in vitro. Minced tissue placed on top of the collagen gel on the right, before plastic compression. Collagen without minced tissue on the left. (b) Scaffold containing minced bladder tissue after plastic compression. (c) Scaffolds containing minced tissue cut to fit in 12-well plates in cell culture. Columns 1 and 2 demonstrating duplicates for urothelium (U), urothelium and detrusor (U + D), and detrusor (D).
