Figure 5.

Morphology of 3D cell expansion on collagen scaffolds after two to three weeks in vitro culture in plastic wells; minced detrusor ((a)–(e)), detrusor and urothelium ((f)–(j)), and urothelium ((k)–(o)), compared to native pig bladder ((p)–(s)). Photomicrographs demonstrating routine staining with haematoxylin-eosin and immunostaining with alpha-actin after two and three weeks, respectively, for cytokeratin (MNF116) and Ki-67. Arrows (j, o, and s) indicating cells with a sustained proliferative capacity (Ki-67).