Figure 6.

Morphology after four weeks in vivo. Transplants with minced detrusor ((a)–(d)), detrusor and urothelium ((e)–(h)), urothelium ((i)–(l)), and control ((m)–(p)). Photomicrographs demonstrating routine histology with haematoxylin-eosin and Masson's trichrome and immunostaining for cytokeratin (MNF116) ((c), (g), and (o)), uroplakin III (k), or alpha-actin, with L indicating the lumen. Regenerated detrusor muscle indicated by (∗), separated from the shivering muscle marked with (†) ((b) and (f)). (j) No regenerated detrusor muscle was shown when transplanting with only minced urothelium. (k) A multilayered continuous transitional epithelium of urothelial origin was confirmed by uroplakin in specimens with urothelium. Immunostaining with alpha-actin demonstrated smooth muscle cells in specimens transplanted with minced detrusor muscle (d) as well as minced detrusor and urothelium (h).