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. 2016 Oct 13;7:1600. doi: 10.3389/fmicb.2016.01600

Figure 3.

Figure 3

ROS accumulation and antioxidant gene expression analysis in PGPR inoculated or non-inoculated salt treated peanut seedlings. (A) DAB and NBT staining for in vivo localization of superoxide and hydrogen peroxide. The upper most leaves (4–5) from 3 to 4 different plants for each treatment was used and the picture of a representative leaf is shown here. (B) Quantitative expression analysis of antioxidant genes, ascorbate peroxidase (APX), catalase (CAT), and superoxide dismutase (SOD). Asterisks (*) indicate significant differences as analyzed by Dunnett's test (P < 0.05). The expression was quantified relative to non-inoculated salt treated seedlings.