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. 2016 Oct 13;9:101. doi: 10.3389/fnmol.2016.00101

Figure 2.

Figure 2

Influence of MPTP-induced DA depletion on neurogenesis in dentate gyrus (DG). (A) Schematic diagram of dorsal DG (d-DG) and ventral DG (v-DG; orange area). (B) Representative pictures of TH+ fibers (white arrows) in d-DG and v-DG on day 7 post-MPTP. Scale bars = 50 μm. (C) Time chart of the experimental procedure. Horizontal open arrow indicates the time after BrdU-injection (day). Vertical open arrows indicate the time of BrdU immuno-staining. (D,E) Representative images of BrdU-positive (BrdU+) cells (black arrows) in d-DG (D) and v-DG (E). Scale bar = 100 μm. The points represent the mean number of D1-, D7-, D14- and D21-BrdU+ cells in d-DG and v-DG. **p < 0.01 vs. control mice (two-way ANOVA). (F) Bar graph shows the mean number of DCX-positive (DCX+) cells in d-DG and v-DG on day 7 post-MPTP. **p < 0.01 vs. control mice (Student’s t-tests). Representative images of DCX+ cells (white arrows). ML: molecular layer; GL: granular cell layer. Scale bar = 50 μm. (G,H) Bar graph shows the mean number of D21-BrdU+/NeuN+ cells and D21-BrdU+/GFAP+ cells in d-DG and v-DG. **p < 0.01 vs. control mice. Representative images of BrdU+/NeuN+ cells (G) and BrdU+/GFAP+ (H) cells (white arrows). Neuronal nuclei (NeuN) and glial fibrillary acidic protein (GFAP) are shown in green and BrdU is shown in red, newborn cells are shown in yellow. Scale bars = 50 μm.