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. 2016 Oct 13;6:34740. doi: 10.1038/srep34740

Figure 4. HBV repressed miR-204 expression through STAT3 activation.

Figure 4

(A) Increased expression of phosphorylated and total protein levels of STAT-3 was detected in stable HBV-producing cells by Western blot with specific antibodies, (B) Treatment with increasing concentrations of a STAT3 inhibitor (S31-201) led to gradual reduction of phosphorylated and total STAT3 protein in stable HBV-producing HepG2 cells. (C) Increasing concentrations of a STAT3 inhibitor (S31-201) led to gradually increased expression of miR-204 as measured by stem-loop Q-PCR. (D) This cartoon summarizes the relationships among HBV, miR-204 and STAT3. The dot line and the question mark represent an unknown relationship between miR-204 and STAT3. Similar expression levels of total and phosphorylated STAT3 protein (E) and mRNA (F) were detected in HepG2 and HuH7 cell lines stably expressing miR-204. (G) A cartoon summarizes a positive feed-forward loop between HBV, miR-204 and STAT3. HBV can reduce the expression of miR-204 through the activation of a STAT3 intermediate.