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. 2016 Oct 13;6:35026. doi: 10.1038/srep35026

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Total extracts from K562C cells untreated (−dox) or treated with doxycycline for 4 days to induce RPS19 depletion (+dox) or from 22Rv1 cells transfected with control (siCNT) or RPS19-specific siRNA (siS19) were analyzed by Western blot with the indicated antibodies. Examples of Western from a single experiment are shown in the upper panels. Quantification of signals from three independent experiments is reported in lower panels as a column plot of the mean ± s.e.m. of the densitometry values normalized by GAPDH. The image of total CDK2 in 22Rv1 cells was obtained by cropping two lanes from a single blot. Uncropped original images are shown in Supplementary information.