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. 2016 Oct 13;6:35026. doi: 10.1038/srep35026

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Total RNA from mononuclear cells of 9 controls or 10 DBA patients harboring different mutations (indicated in Table 1) were analyzed by RT-qPCR with primers specific for 47S and β-actin. The results of triplicate RT-qPCR analyses from each sample are reported as a dot plot of 47S rRNA level calculated with the 2^−ΔCt method. The orizontal bars indicate the means of the two groups of values.