Table 1. 3, 4-DHSA dioxygenases used for constructing the phylogenetic tree shown in Fig. S4.
| Microorganism | Group | Sequence identity (%) | Accession |
|---|---|---|---|
| Comamonas testosteroni TA441 | β-proteobacteria | 100.0% | Q9FAE3 |
| Pseudomonas putida DOC21 | γ-proteobacteria | 62.2% | H9ZGL6 |
| Shewanella pealeana ATCC 700345 | γ-proteobacteria | 61.7% | A8H4I6 |
| Pseudoalteromonas haloplanktis TAC 125 | γ-proteobacteria | 59.7% | Q3IE83 |
| Burkholderia cenocepacia J2315 | β-proteobacteria | 59.1% | B4EKN7 |
| Ralstonia eutropha (Cupriavidus necator) H16 | β-proteobacteria | 58.8% | F8GRC0 |
| Cupriavidus taiwanensis LMG 19424 | β-proteobacteria | 58.4% | B2AIU1 |
| Rhodococcus jostii RHA1 | actinobacteria | 44.5% | Q9KWQ5 |
| Gordonia polyisoprenivorans DSM 44266 | actinobacteria | 44.1% | H6MSZ4 |
| Amycolicicoccus subflavus DSM 45089 | actinobacteria | 44.1% | F6EN61 |
| Amycolatopsis decaplanina DSM 44594 | actinobacteria | 44.0% | M2WZF0 |
| Amycolatopsis mediterranei U-32 | actinobacteria | 43.8% | D8HWH1 |
| Kutzneria sp. 744 | actinobacteria | 43.3% | W7SSZ6 |
| Rhodococcus pyridinivorans AK37 | actinobacteria | 43.1% | H0JLI7 |
| Gordonia terrae C-6 | actinobacteria | 42.5% | R7YBG9 |
| Dietzia cinnamea P4 | actinobacteria | 42.2% | E6J6R1 |
| Mycobacterium tuberculosis ATCC 25618 | actinobacteria | 42.1% | I6YCG0 |
| Nocardia seriolae | actinobacteria | 41.3% | A0A0B8MZT4 |
| Pseudomonas pseudoalcaligenes* | γ-proteobacteria | 39.7% | P08695 |
The sequences were retrieved from the UniProtKB/TrEMBL database by performing standard BLASTP search with 3, 4-DHSA dioxygenase (the product of tesB) from C. testosteroni TA441 as a query. In known steroid-degrading proteobacteria, the dioxygenase gene is harbored in the conserved testosterone-degradation gene cluster25.
*The biphenyl-2, 3-diol 1, 2-dixoxygenase of P. pseudoalcaligenes.