Skip to main content
. 2016 Oct 13;6:35014. doi: 10.1038/srep35014

Figure 3. Accelerated onset of colitis in Lcn2/IL-10 DKO mice compared to IL-10 KO mice.

Figure 3

(a) Representative macroscopic findings of the colons of IL-10 KO and Lcn2/IL-10 DKO mice at 4 weeks of age. (b) Representative HE-staining images (left) and histological scores (right) of the colonic tissues of IL-10 KO and Lcn2/IL-10 DKO mice at 4 weeks of age. Scale bars, 100 μm. N = 9 for each group. Error bars represent SD. *P < 0.05 compared with IL-10 KO mice by non-parametric Mann-Whitney U test. (c–e) Gene expression of IL-1β, TNF-α, IFN-γ, and IL-17A in the colonic tissues (c), TNF-α and IL-12p40 secretion from colonic explants (d), and IFN-γ and IL-17A secretion from unseparated MLN cells stimulated with CBLs (e), of IL-10 KO and Lcn2/IL-10 DKO mice at 4 weeks of age. Gene expression of each target molecule was normalized to GAPDH. N = 7–8 per group. The box refers to the interquartile range and the bar inside represents the median. *P < 0.05 compared with IL-10 KO mice by non-parametric Mann-Whitney U test. MLN, mesenteric lymph-node. CBLs, cecal bacterial lysates. N.S., not significant.