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. 2016 Oct 13;6:35373. doi: 10.1038/srep35373

Figure 7. Expression of cytokines and innate immunity receptors following Polyman26 stimulation.

Figure 7

iMDDCs from 4 separate donors were treated with medium culture, Polyman26 (10 μM), LPS (1 μg/ml), or Polyman26+ LPS for 3, 24, and 48 h. (a) Gene expression following 3 h treatment. For each condition, cDNA was pooled and gene expression evaluated by Real-time PCR array. Expression was shown as fold induction relative to unstimulated control. Targets showing at least a 2-fold modulation were considered significant. (b–e) Cytokine production. The concentration of Il-1β (b), IL-6 (c), TNFα, (d), and IL-12 (e) in the culture supernatants was assayed by ELISA at 24 and 48 h. (b-e) Values represent the mean ± SD. *p < 0.05; **p < 0.01 (Student’s t-test).