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High-throughput fluorescence-based thermal shift assays of PscD-SD with 190 potential ligands from Biolog plates PM1 (a) and PM2 (b). Each histogram indicates the ΔTm, calculated as the Tm in the presence of ligand minus the Tm in the absence of ligand. The ligands identified as potential hits are labelled. The values graphed are the mean and standard error from three independent experiments.
