Fig. 1.

Distributions of true scaled selection coefficients, S, for a representative natural simulation (1GV3_A) and each DMS simulation. Scaled selection coefficients have been binned at $S\ge 10$ and $S\le -10$ for visualization. Histograms depicting the true S distribution for all other natural simulations are provided in supplementary figures S8–S10, Supplementary Material online. S distributions shown represent the selection coefficients among all possible single-nucleotide changes, across all sites.