Figure 7.

Enzymatic activity of CA XII proteins bearing p.His121Gln or p.Glu143Gly substitutions. Enzymatic activity of CA XII mutants p.His121Gln and p.Glu143Gly was determined by assaying the reversible rate of hydration of CO₂ as previously described in the absence (left) or presence (right) of physiological concentration of NaCl and normalizing to wild-type. Boxes represent the interquartile range (IQR) and the horizontal bars within the boxplots represent the median. The top whisker represents the 75th percentile plus 1.5 times the IQR and the bottom whisker represents the 25th percentile minus 1.5 times the IQR. Circles represent statistical outliers. (Boxplot statistics calculated in R.).