Figure 3.

ASC-JM17 increases the expression of antioxidant enzymes via Nrf2. (A) Expression of antioxidant enzymes in PC12 cells following treatment with indicated concentrations of ASC-JM17. (B) PC12 cells were transfected with indicated siRNAs, treated with ASC-JM17 and analyzed by western blotting. (C) PC12 cells were treated with indicated concentrations of ASC-JM17 for 16 h, or with 1 mm H₂O₂ for 30 min, which induces oxidative stress in cells. The oxidation status of total cellular proteins was visualized by western blotting after derivatization with 2,4-dinitrophenyl hydrazine. HO-1 protein levels were also analyzed. (D) MCF7 cells transfected with indicated siRNAs were pretreated with 2.5 µm ASC-JM17 for 16 h, followed by indicated concentrations of H₂O₂ for 4 h. Cell viability was assessed using the XTT assay. Data are expressed as mean ± SEM (n = 3). **P < 0.01 (one-way ANOVA).