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Immunofluorescence analysis of retinal cryosections from 1-month-old mice of indicated genotypes was performed using anti-rhodopsin (green) or anti-M-opsin (red) antibodies. Nuclei were stained with Hoechst (blue). Arrows indicate mislocalized opsins in the outer nuclear layer (ONL) and outer plexiform layer (OPL). Scale bars: 40 μm. OS, outer segment; IS, inner segment. Abundant mislocalized M-opsin is also detected in the IS of the Rpgrko/Y::Cep290rd16/+ mice.
