Figure 1. IHC of xenografts.

Tumour xenografts consisting of TN MDA-MB-231 breast cancer cells co-transplanted with primary human monocytes, express more myeloid-related and immunosuppressive markers than luminal A MCF-7/monocyte xenografts. The xenografts were grown in highly immunodeficient NSG-mice (see ‘Methods' section), and sections from the tumours were stained with myeloid (CD163, CD11b, S100A9) tumour (vimentin) and endothelial markers (CD34). The two cell lines chosen are negative for S100A9 (ref. ²¹). IHC was performed using the indicated antibodies. All histological sections were counterstained with HE. N=5 mice were analysed for each group; MCF-7 grafts were analysed on day 21 and 90 post-graft with similar results—day 90 is shown here; TN MDA-MB-231 grafts were grown to day 21 only. The histograms show the mean value for each IHC score with statistical analyses. IHC scores are shown in Supplementary Table 1. *=P<0.05, **=P<0.01 ANOVA non-parametric Kruskal–Wallis test. N=5. Error bars indicate s.e.m.