Figure 3. ETI-mediated early induction of JA-responsive genes is dependent on SA and NPR3 and NPR4 but independent of NPR1 and the JA receptor COI1.

Leaves from corresponding plants were harvested 4 h.p.i. with Psm ES4236/avrRpt2 (avrRpt2) at OD_600nm=0.2 or 10 mM MgSO₄ (Control). qRT-PCR was performed on LOX3, JAZ1, JAZ10, with UBQ5 as a reference. Gene expression (a) in WT, sid2 and rps2; (b) in WT, npr1 and rps2; (c) in WT, npr3 npr4 (n3n4), npr1 npr3 npr4 (n1n3n4) and rps2; (d) in WT, coi1 and rps2 was measured. Data from three biological replicates were combined using linear mixed-effects model. Significant difference was detected using Student's t-test. Data are shown as mean±s.d. *P<0.05; **P<0.01; ***P<0.001; NS, no significant difference. qRT-PCR, quantitative reverse transcription PCR.