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. 2016 Oct 11;7:13085. doi: 10.1038/ncomms13085

Figure 4. SAXS model of the Nop9 protein and ITS1 D-A2 subdomain A pre-rRNA.

Figure 4

(a) Rigid body SAXS model of the Nop9:ITS1 D-A2 subdomain A pre-rRNA complex. The crystal structure of Nop9 and a predicted model of the ITS1 D-A2 Subdomain A pre-rRNA from SAXS data of the RNA alone are shown. Positively and negatively charged surfaces are shown in blue and red, respectively. The vacuum electrostatic surface potential was calculated using the APBS module within PyMol. (b) ITS1 D-A2 pre-rRNA contacts the conserved Nop9 repeats. The extent of amino-acid sequence conservation was calculated using the ConSurf server53 with default settings, and the results are displayed on a ribbon diagram of Nop9 (highly conserved residues are coloured maroon and less conserved residues are cyan). The most highly conserved residues are shown with space-filling spheres.