Fig. 3.
Comparison of the effects of different TRIM proteins on the early stages of HIV-1 replication. a, b The indicated HEK293 cell lines were infected with 50 ng/ml (p24gag) HIV-1 and viral transduction was analyzed by luciferase activity (a) and late reverse transcripts were measured at 24 h post infection by qPCR (b). c The indicated HEK293 cell lines were infected with HIV-1 for 3 h. Cell lysates were processed for the fate-of-capsid assay as described in Fig. 2c. The levels of p24 in pelletable and input fractions were measured by densitometry and pellet/input ratio was calculated. Error bars represent the standard deviations from three independent replicates. *P < 0.05