Fig. 4.
Disruption of microtubule dynamics aborts TRIM11-mediated HIV-1 premature disassembly and reduction of reverse transcription levels. a, b The indicated HEK293 cell lines were treated with DMSO, 10 μM MG132 (a) or 10 μM CQ (b) for 3 h before infection with HIV-1. Three hours post infection, cell lysates were processed for the fate-of-capsid assay as described in Fig. 2c. c, d The indicated cell lines were infected with HIV-1 in the presence of DMSO, 0.1 μM nocodazole (c) and 0.1 μM taxol (d) for 3 h. Cell lysates were processed for the-fate-of capsid assay as described in Fig. 2c. e, f HEK293 cells expressing TRIM11-HA and vector (e), HEK293 cells stably knocked down TRIM11 (f) were infected with 50 ng/ml (p24gag) HIV-1 in the presence of DMSO, 0.1 μM nocodazole and 0.1 μM taxol and analyzed by qPCR for late reverse transcripts at 3 h post infection. Error bars represent the standard deviations from three independent replicates. *P < 0.05