Fig. 5.

(A) Representative regions of cell images shown for DAPI, HA-mAPJ and an illustration of the automated image segmentation used to define perimeters of nuclei (blue) and cells (green or red) and inclusions (yellow) in cells stimulated with control or [Pyr¹]apelin-13, as indicated. (B) Time course of [Pyr¹]apelin-13–induced HA-mAPJ internalization. HA-mAPJ-HEK293 cells were pre-treated with anti-HA antibody (1:1000; 1 h 37 °C/5% CO₂), washed, then incubated in the presence or absence of [Pyr¹]apelin-13 (100 nM) for 0–6 h. Data shown are mean ± SEM, of at least three separate experiments, each with triplicate wells and triplicate fields within wells. **p < 0.01, comparing stimulations to basal conditions, analysed by one-way ANOVA and Dunnett's multiple comparison post hoc tests. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)