Skip to main content
. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Cell. 2016 Sep 8;167(1):47–59.e15. doi: 10.1016/j.cell.2016.08.028

Figure 6. Anterograde tracing reveals downstream thermoregulatory circuit.

Figure 6

(A) Schematic summarizing strategy for visualizing target structures using Cre-dependent virus expressing a synaptophysin-mCherry fusion protein. Major VMPOPACAP neuron projection sites are indicated.

(B) Expression of synaptophysin-mcherry (dark) in VMPOPACAP neurons cell bodies at VMPO injection site.

(C) Expression of synaptophysin-mcherry in VMPOPACAP neuron terminals at indicated regions (red squares). Abbreviations: LSV- ventral portion of lateral septum; BNST- bed nucleus of stria terminalis. DMH- dorsomedial hypothalamus; ARC-arcuate nucleus; PVT-paraventricular thalamus; MHb-medial habenula; PVH-paraventricular hypothalamus; vlPAG- ventrolateral periaqueductal gray. Scale bars = 100 µm.