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. Author manuscript; available in PMC: 2017 Sep 22.
Published in final edited form as: Cell. 2016 Sep 8;167(1):47–59.e15. doi: 10.1016/j.cell.2016.08.028
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies
Rabbit Polyclonal anti-Phospho-Ribosomal protein S6
pSer244/pSer247
Invitrogen Cat# 44-923G
RRID:AB_2533798
Mouse monoclonal anti-GFP Memorial-Sloan
Kettering Monoclonal
Antibody Facility
Htz-GFP-19F7
Htz-GFP-19C8
Chicken polyclonal anti-GFP Abcam Cat# ab13970
RRID:AB_300798
Rat monoclonal anti-RFP Chromotek Cat# rfp-antibody-
5f8
RRID:AB_2336064
Mouse monoclonal anti-galactosidase Beta Promega Cat# Z378A
RRID:AB_2313752
Mouse monoclonal anti-NeuN Millipore Millipore Cat#
MAB377,
RRID:AB_2298772
Chemicals, Peptides, and Recombinant Proteins
Icilin Sigma-Aldrich Cat# I9532; CAS:
36945-98-9
Capsaicin Calbiochem EMD
Millipore
Cat# 211275; CAS:
404-86-4
Clozapine-N-oxide Tocris Bioscience Cat# 4936; CAS:
34233-69-7
Critical Commercial Assays
Ovation RNA-Seq System V2 Nugen Cat#7102-08
Ovation Ultralow DR Multiplex system Nugen Cat#0330
RNAscope Manual Fluorescent Multiplex kit Advanced Cell
Diagnostics
Cat#320293/320513
Deposited Data
PhosphoTRAP RNA-Seq raw and analysed data This paper GEO: GSE80121
TRAP-Seq RNA-Seq raw and analysed data This paper GEO: GSE80120
Experimental Models: Cell Lines
Experimental Models: Organisms/Strains
Mouse: PACAP-2A-Cre/Adcyap1-2A-Cre Allen Institute for Brain
Science
NA
Mouse: BDNF-2A-Cre This paper NA
Mouse: Gal-Cre: B6.FVB(Cg)-Tg(Gal-
cre)K187Gsat/Mmucd
Mutant Mouse
Resource and
Research Centers
031060-UCD
Mouse: Vglut2-IRES-Cre: Slc17a6tm2(cre)Lowl The Jackson
Laboratory
016963
Mouse: Gad2-2A-nls-mcherry: B6;129S-Gad2tm1.1Ksvo/J The Jackson
Laboratory
023140
Mouse: Nos1-IRES-Cre: B6.129-Nos1tm1(cre)Mgmj/J The Jackson
Laboratory
017526
Mouse: BDNF-LSL-LacZ: B6.129S2(Cg)-Bdnftm1Krj/J The Jackson
Laboratory
021055
Mouse: Rosa26-LSL-GFPL10a: B6.129S4-
Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp/J
The Jackson
Laboratory
022367
Recombinant DNA
AAV2- EF1a-DIO-EGFP-L10a This Paper (packaged
by UNC vector core)
NA
AAV1-CAG-DIO-GCAMP6s Upenn Vector Core NA
AAV5- hEF1α -DIO-hChR2(C128S/D156A)-eYFP
[SSFO]
UNC Chapel Hill
Vector Core
NA
AAV5- EF1a- DIO-hChR2(H134R)-eYFP UNC Chapel Hill
Vector Core
NA
AAV9-hEF1α- DIO-Synaptophysin-mCherry MIT McGovern Viral
Core Facility
NA
HSV-hEF1α-LSL-HAFlagL10a-WPRE-SV40 This paper (packaged
by MIT McGovern
Viral Core)
NA
AAV2-EF1a-DIO-hM3Dq-mcherry UNC Chapel Hill
Vector Core
NA
Sequence-Based Reagents
EGFP In-situ hybridisation probe Advanced Cell
Diagnostics
400281-C3
mm-BDNF In-situ hybridisation probe Advanced Cell
Diagnostics
424821
BDNF sgRNA sequence:
CCATTAAAAGGGGAAGATAGGTTTAAGAGCTATGCT
GGAAACAGCATAGCAAGTTTAAATAAGGCTAGTCCG
TTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTT
TTTTT
This Paper NA
BDNF-2A-Cre genotyping primers:
Fwd: TCAATACCGGAGATCATGCAAGC
Rev: CTGCTGCCATGCATAAAACATT
This Paper NA
Software and Algorithms
ArrayStar® Version 11.0 DNASTAR Inc. NA
pClamp 10.5 Molecular Devices NA
GraphPad Prism 7.0 GraphPad NA
Other