REAGENT or RESOURCE | SOURCE | IDENTIFIER |
---|---|---|
Antibodies | ||
Rabbit Polyclonal anti-Phospho-Ribosomal protein S6 pSer244/pSer247 |
Invitrogen | Cat# 44-923G RRID:AB_2533798 |
Mouse monoclonal anti-GFP | Memorial-Sloan Kettering Monoclonal Antibody Facility |
Htz-GFP-19F7 Htz-GFP-19C8 |
Chicken polyclonal anti-GFP | Abcam | Cat# ab13970 RRID:AB_300798 |
Rat monoclonal anti-RFP | Chromotek | Cat# rfp-antibody- 5f8 RRID:AB_2336064 |
Mouse monoclonal anti-galactosidase Beta | Promega | Cat# Z378A RRID:AB_2313752 |
Mouse monoclonal anti-NeuN | Millipore | Millipore Cat# MAB377, RRID:AB_2298772 |
Chemicals, Peptides, and Recombinant Proteins | ||
Icilin | Sigma-Aldrich | Cat# I9532; CAS: 36945-98-9 |
Capsaicin | Calbiochem EMD Millipore |
Cat# 211275; CAS: 404-86-4 |
Clozapine-N-oxide | Tocris Bioscience | Cat# 4936; CAS: 34233-69-7 |
Critical Commercial Assays | ||
Ovation RNA-Seq System V2 | Nugen | Cat#7102-08 |
Ovation Ultralow DR Multiplex system | Nugen | Cat#0330 |
RNAscope Manual Fluorescent Multiplex kit | Advanced Cell Diagnostics |
Cat#320293/320513 |
Deposited Data | ||
PhosphoTRAP RNA-Seq raw and analysed data | This paper | GEO: GSE80121 |
TRAP-Seq RNA-Seq raw and analysed data | This paper | GEO: GSE80120 |
Experimental Models: Cell Lines | ||
Experimental Models: Organisms/Strains | ||
Mouse: PACAP-2A-Cre/Adcyap1-2A-Cre | Allen Institute for Brain Science |
NA |
Mouse: BDNF-2A-Cre | This paper | NA |
Mouse: Gal-Cre: B6.FVB(Cg)-Tg(Gal- cre)K187Gsat/Mmucd |
Mutant Mouse Resource and Research Centers |
031060-UCD |
Mouse: Vglut2-IRES-Cre: Slc17a6tm2(cre)Lowl | The Jackson Laboratory |
016963 |
Mouse: Gad2-2A-nls-mcherry: B6;129S-Gad2tm1.1Ksvo/J | The Jackson Laboratory |
023140 |
Mouse: Nos1-IRES-Cre: B6.129-Nos1tm1(cre)Mgmj/J | The Jackson Laboratory |
017526 |
Mouse: BDNF-LSL-LacZ: B6.129S2(Cg)-Bdnftm1Krj/J | The Jackson Laboratory |
021055 |
Mouse: Rosa26-LSL-GFPL10a: B6.129S4- Gt(ROSA)26Sortm1(CAG-EGFP/Rpl10a,-birA)Wtp/J |
The Jackson Laboratory |
022367 |
Recombinant DNA | ||
AAV2- EF1a-DIO-EGFP-L10a | This Paper (packaged by UNC vector core) |
NA |
AAV1-CAG-DIO-GCAMP6s | Upenn Vector Core | NA |
AAV5- hEF1α -DIO-hChR2(C128S/D156A)-eYFP [SSFO] |
UNC Chapel Hill Vector Core |
NA |
AAV5- EF1a- DIO-hChR2(H134R)-eYFP | UNC Chapel Hill Vector Core |
NA |
AAV9-hEF1α- DIO-Synaptophysin-mCherry | MIT McGovern Viral Core Facility |
NA |
HSV-hEF1α-LSL-HAFlagL10a-WPRE-SV40 | This paper (packaged by MIT McGovern Viral Core) |
NA |
AAV2-EF1a-DIO-hM3Dq-mcherry | UNC Chapel Hill Vector Core |
NA |
Sequence-Based Reagents | ||
EGFP In-situ hybridisation probe | Advanced Cell Diagnostics |
400281-C3 |
mm-BDNF In-situ hybridisation probe | Advanced Cell Diagnostics |
424821 |
BDNF sgRNA sequence: CCATTAAAAGGGGAAGATAGGTTTAAGAGCTATGCT GGAAACAGCATAGCAAGTTTAAATAAGGCTAGTCCG TTATCAACTTGAAAAAGTGGCACCGAGTCGGTGCTT TTTTT |
This Paper | NA |
BDNF-2A-Cre genotyping primers: Fwd: TCAATACCGGAGATCATGCAAGC Rev: CTGCTGCCATGCATAAAACATT |
This Paper | NA |
Software and Algorithms | ||
ArrayStar® Version 11.0 | DNASTAR Inc. | NA |
pClamp 10.5 | Molecular Devices | NA |
GraphPad Prism 7.0 | GraphPad | NA |
Other |