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. 2016 Jun 20;44(18):8951–8961. doi: 10.1093/nar/gkw564

Figure 5.

Figure 5.

Loss of U2345 pseudouridylation causes no changes in cell growth and ribosome synthesis. (A) Cell growth study. Serial 10-fold dilutions of the isogenic wild-type BY5208 and ΔsnR9 strains grown at 19°C for 48 h or 30°C and 37°C for 24 h were spotted onto YPD-containing agar plates. (B) Ribosome profiles of BY5208 (upper panel) and ΔsnR9 (lower panel) strains. Ribosomal and polysomal fractions prepared from the wild-type and ΔsnR9 strains (each cultured at 30°C for 24 h in YPD medium) were subjected to sucrose density gradient centrifugation. A254 was monitored.