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. 2016 Jun 30;44(18):8962–8975. doi: 10.1093/nar/gkw572

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Quantitation of dN misincorporation in coding and non-coding RNAs. dNrN dinucleotides were quantified by alkaline hydrolysis LC-MS/MS in different RNA species. (A) Human CCRF-SB cells: poly(A) RNA, rRNA and tRNA. (B) E. coli DH5α rRNA and tRNA. (C) DENV-2 virus RNA genome. Values represent mean ± SD for three independent cultures for CCRF-SB and DH5α, and for three technical replicates of a single culture for DENV-2.