Table 2. Levels of dNs in cellular and viral RNA species1.
| Organism | RNA species | dC per 106 nt | dT per 106 nt | dA per 106 nt | dG per 106 nt | Total dN/106 nt | Polymerase |
|---|---|---|---|---|---|---|---|
| Human CCRF-SB | poly(A) RNA | 2.1 ± 0.6 | 0.7 ± 0.5 | 5.3 ± 1.0 | 8.9 ± 1.4 | 17 ± 3.5 | Pol II + PAP + TUTase |
| 28S rRNA | 0.9 ± 0.2 | 0.2 ± 0.06 | 0.3 ± 0.06 | 1.1 ± 0.3 | 2.6 ± 0.6 | Pol I | |
| 18S rRNA | 1.6 ± 0.6 | 0.2 ± 0.08 | 0.6 ± 0.1 | 1.0 ± 0.4 | 3.3 ± 1.3 | Pol I | |
| 5.8S rRNA | 2.8 ± 0.8 | 0.5 ± 0.3 | 0.8 ± 0.3 | 1.2 ± 0.5 | 5.3 ± 1.8 | Pol I | |
| 5S rRNA | 4.3 ± 0.6 | 0.6 ± 0.09 | 0.9 ± 0.3 | 5.3 ± 0.6 | 11 ± 2 | Pol III | |
| tRNA | 6.3 ± 1.4 | 1.0 ± 0.1 | 0.8 ± 0.3 | 2.4 ± 0.4 | 11 ± 2 | Pol III + NTR | |
| S. cerevisiae W1588-4C | poly(A) RNA | 12 ± 3 | 8.9 ± 2.1 | 2000 ± 950 | 1100 ± 440 | 3200 ± 1400 | Pol II + PAP + TUTase |
| 25S rRNA | 6.1 ± 2.8 | 16 ± 4 | 96 ± 18 | 132 ± 58 | 250 ± 83 | Pol I + PAP | |
| 18S rRNA | 8.9 ± 1.9 | 12 ± 0.8 | 85 ± 5 | 55 ± 12 | 161 ± 20 | Pol I + PAP | |
| 5.8S rRNA | 10 ± 3 | 4.6 ± 0.6 | 64 ± 5 | 87 ± 13 | 165 ± 21 | Pol I + PAP | |
| 5S rRNA | 19 ± 4 | 6.7 ± 1.4 | 55 ± 8 | 96 ± 23 | 176 ± 36 | Pol III + PAP | |
| tRNA | 43 ± 11 | 17 ± 1 | 90 ± 13 | 128 ± 38 | 278 ± 63 | Pol III + NTR + PAP | |
| S. cerevisiae W1588-4C + MMS | poly(A) RNA | 23 ± 5 | 15 ± 2 | 1,200 ± 260 | 697 ± 169 | 2000 ± 440 | Pol II + PAP + TUTase |
| 25S rRNA | 32 ± 6 | 21 ± 4 | 90 ± 6 | 132 ± 16 | 276 ± 33 | Pol I + PAP | |
| 18S rRNA | 26 ± 7 | 14 ± 2 | 100 ± 22 | 136 ± 16 | 276 ± 47 | Pol I + PAP | |
| 5.8S rRNA | 26 ± 6 | 7.2 ± 1.4 | 64 ± 7 | 71 ± 13 | 168 ± 28 | Pol I + PAP | |
| 5S rRNA | 47 ± 18 | 10 ± 2 | 50 ± 8 | 182 ± 79 | 290 ± 108 | Pol III + PAP | |
| tRNA | 157 ± 44 | 34 ± 5 | 116 ± 9 | 357 ± 57 | 663 ± 115 | Pol III + NTR + PAP | |
| E. coli DH5α | 23S rRNA | 31 ± 8 | 12 ± 2 | 9.9 ± 3.8 | 54 ± 13 | 108 ± 27 | RNAP |
| 16S rRNA | 19 ± 8 | 13 ± 5 | 11 ± 2 | 50 ± 23 | 93 ± 38 | RNAP | |
| 5S rRNA | 24 ± 17 | 6.8 ± 5.2 | 10 ± 7 | 36 ± 18 | 76 ± 47 | RNAP | |
| tRNA | 32 ± 12 | 22 ± 4 | 12 ± 6 | 36 ± 10 | 101 ± 32 | RNAP | |
| E. coli W3110 | 23S rRNA | 54 ± 5 | 6.3 ± 0.6 | 7.5 ± 0.7 | 44 ± 3 | 111 ± 9 | RNAP |
| 16S rRNA | 45 ± 8 | 5.0 ± 0.8 | 5.4 ± 1.3 | 37 ± 11 | 92 ± 21 | RNAP | |
| 5S rRNA | 65 ± 14 | 5.3 ± 1.4 | 6.4 ± 1.0 | 41 ± 7 | 118 ± 23 | RNAP | |
| tRNA | 65 ± 7 | 7.2 ± 1.2 | 4.1 ± 1.2 | 31 ± 4 | 107 ± 14 | RNAP | |
| E. coli W3110 Δndk | 23S rRNA | 203 ± 32 | 9.4 ± 0.7 | 5.1 ± 1.0 | 70 ± 19 | 288 ± 53 | RNAP |
| 16S rRNA | 167 ± 37 | 8.5 ± 0.9 | 5.4 ± 1.4 | 32 ± 4 | 213 ± 43 | RNAP | |
| 5S rRNA | 345 ± 51 | 8.5 ± 2.0 | 8.0 ± 1.2 | 55 ± 7 | 416 ± 61 | RNAP | |
| tRNA | 157 ± 46 | 9.9 ± 2.6 | 3.5 ± 0.4 | 26 ± 5 | 196 ± 54 | RNAP | |
| DENV-2 virus | RNA genome | 171 ± 36 | 39 ± 21 | 103 ± 31 | 455 ± 95 | 768 ± 183 | RdRP |
1Values (as dN per 106 nt) represent mean ± SD for three independent cultures for all cell lines, and for three technical replicates of a single culture for DENV-2 virus. PAP, poly(A) polymerase; TUTase, 3′ terminal uridylyl transferase; NTR, tRNA nucleotidyltransferase.