Figure 1.

CRISPRi of sigH and pknB in M. smegmatis. (A) Position of target sequences, shown by purple vertical lines. Numbering refers to the first nucleotide in the target sequence relative to annotated start of coding sequence. Circled sgRNAs are those for which expression data are shown. The arrow shows the position of the transcription start site. (B and D) Expression of dcas9 determined by RT-qPCR with and without induction of dcas9 and the sgRNA indicated. (C and E) Expression of pknB and sigH, respectively, with and without induction. The significance of the difference in gene-specific RNA between samples from induced and uninduced cultures is indicated by asterisks above the bar for the induced sample (*P < 0.01; **P < 0.001; ***P < 0.0001).