Figure 4.

CRISPRi of wag31 and ftsZ in M. tuberculosis. (A, D, G, J) Expression of dcas9 with and without induction of dcas9 and the sgRNA indicated. (B, E, H, K) Expression of wag31 or ftsZ with and without induction of dcas9 and the sgRNA indicated. (C, F, I, L) Growth curves of culture with and without induction of dcas9 and the sgRNA indicated. The significance of the difference in gene-specific RNA between samples from induced and uninduced cultures is indicated by asterisks above the bar for the induced sample (*P < 0.01; **P < 0.001; ***P < 0.0001).