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. 2016 Jul 12;44(18):e143. doi: 10.1093/nar/gkw625

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© The Author(s) 2016. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 6. — Titration of inducer for CRISPRi of ftsZ in M. tuberculosis. (A) Expression of dcas9 at 24 and 48 h of induction with different concentrations of aTc. (B) Expression of ftsZ at 24 and 48 h of induction with different concentrations of aTc. The significance of the difference in gene-specific RNA in samples from cultures treated with different concentrations of aTc compared to untreated samples at the same time point is indicated by asterisks above the bar for the induced samples (*P < 0.01; **P < 0.001; ***P < 0.0001).

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